Bioethanol production part 2

Based on the results obtained in a period of 5 years, it will be possible to implement a fully functional, economic feasible and international competitive bioethanol plant in DK. The overall project is split in 4 parts of which this is the second and includes: Design of an optimized protocol for wet oxidation of combined manure and wheat straw, including chemical analysis of carbohydrates, inorganic salts, and organic acids. Development and testing of a lab-scale wet explosion reactor for pre-treatment. Assessment of the potential enzymatic hydrolysis of pre-treated hydrolyzate using newly developed enzyme preparations and enzymatic active strain found by screening of existing strain collections. Designing genetic tools for improvement of thermophilic, ethanol producing bacteria for development of strains with deficient acetate and lactate production in order to optimize the ethanol yield from fermentation of the sugars enclosed in the lignocellulosic biomass. Evaluation of the prospects of removing inhibitory compounds in order to maximise the recirculation of process water, and determination of the biogas potential from other various steps in the biomass-to-ethanol process